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Research Laboratories

APPLIED MICROBILOGY LAB

DEPARTMENT OF FORESTRY

The Applied Microbiology lab was established in 2018 and supervised by Dr. Madhu Kamle and Dr. Pradeep Kumar (Lien) and a specialized laboratory that emphasizes on the practical aspects of microbiology. The Lab focuses research areas are applied microbiology, biocontrol, phytochemistry, tissue culture and nanotechnology. The applied microbiology lab serves as a hub for applied research, where researchers explore the potential of microorganisms for various applications, develop innovative solutions and contribute to advancements in fields like forests, agriculture and human and environmental health. The lab has the equipped with the basic amenities required for research work and all the equipment’s and chemicals available were all purchased under nationally funded projects. At present, there are five Ph.D. scholars in the lab and laboratory also trained twelve M.Sc. and six M. Tech students for their research work on different research areas. During last five years published more than 50 research paper in reputed peer reviewed journals like 3Biotech, Frontiers in microbiology, Frontiers in Nutrition Scientific reports, Phytotherapy research, Toxins, Toxicon, International Journal of Food Science and technology, Plants, Nutrition, Food chemistry, Egypt Journal of Biological Pest Control etc. 06 Books (Springer Nature, Taylor & Francis, CRC press) and more than 20 book chapters (Springer Nature, Taylor & Francis, Wiley, Elsevier). Currently two project ongoing first “Use of non-toxic nano formulations for prolonging shelf life and reduction of post-harvest loss of Mandarin orange (Citrus reticulata) of North East India” funded by DBT under this project worked on development of eessential oils based and nanoformulations of non-toxic coating material, safe and effective solution for controlling decay and preserving the quality of fresh crops. We also investigate the effects of essential oils on the quality and shelf life of citrus variety and improving the various parameters meters such as pH, titratable acidity, total phenolic content (TPC), total flavonoid content (TFC), weight loss percentage, decay percentage and prolonged shelf life of the lemons. Second “Screening of Phytochemical and Bioactive compounds against human pathogenic bacteria from selected medicinal indigenous plants of Arunachal Pradesh, India” under this project we exploring the traditional indigenous medicinal plants of Arunachal Pradesh for their phytochemicals and bioactive molecules for the reliable source of plant pharmaceuticals and testing their antimicrobial properties against human pathogenic bacteria. Two projects completed recently: i. “Exploring the Bacillus thuringiensis genetic diversity with anti-coleopteran and anti-lepidopteran specific Cry gene to combat agriculturally important insect pest from North East (Arunachal Pradesh) India” funded by SERB under this project isolation of B. thuringiensis and identification of genes against agriculturally important anti-coleopteran and anti-lepidopteran insect pest. ii. “Micropropagation of Litsea cubeba (Lour.) Pers: an indigenous plant of North East India for genetic conservation” (Completed). Funded by GBPNIHESD under this project we are standardized the protocol for the micropropagation of Litsea cubeba a very significantly important medicinal plants of Arunachal Pradesh. The lab is equipped with state-of-the-art equipment and facilities to facilitate research and experimentation. It typically includes

  1. Laminar Air Flow
  2. Autoclave
  3. Incubators
  4. Microscope
  5. Hot Air Oven
  6. Spectrophotometer
  7. Centrifuge
  8. Ultra Freezer -80°
  9. 4° refrigerator
  10. Rotary Evaporator
  11. Plant Growth Chamber
  12. Microbe culture facilities
  13. Gel units (Vertical and Horizontal)
  14. Weighing Machines
  15. Vortex & pH Meter
  16. Magnetic Stirrer

Quality control and safety: The lab adheres to strict quality control measures to ensure the accuracy and reliability of experimental results. It follows standard operating procedures (SOPs) for handling microorganisms, maintaining sterile conditions and preventing cross contamination. It also ensures compliance with biosafety regulations and practices to protect researchers and the environment from hazardous microorganisms.

Last year publications 2022-23 (Papers, Book Chapters & Book)

  1. Sarkar S, Kamle M, Bharti A, Kumar P* (2023) Antibiotic-Resistant Bacteria Risks and Challenges for Human Health and Environment: An Overview. World J Environ Biosci.12(2):26-34. 
  2. Gogoi P, Kamle M*, Kumar P. (2023) Endophytic Bacteria Associated with Rice: Role in Biotic and Abiotic Stress Protection and Plant Growth Promotions. World J Environ Biosci. 12(1):1-9.  
  3. Bora H, Kamle M, Hassan H, Al-Emam A, Chopra S, Kirtipal N, Bharadwaj S, Kumar P*. (2022) Exploration of Potent Antiviral Phytomedicines from Lauraceae Family Plants against SARS-CoV-2 Main Protease. Viruses. 2022; 14(12):2783. (IF 5.85).
  4. Bora H, Kamle M, Chopra S, Kumar P* (2022). Evaluation of Phytochemical Components, Antioxidant, and Antibacterial Activities of Coptis teeta Walls. Int J Pharm Res Allied Sci. 11(4):140-50.  (IF 0.41).
  5. Bora H, Kamle M, Kumar P* (2022) Phytochemical Analysis, Antioxidant and Antimicrobial Screening of Leaves Extract of Tetrastigma thomsonianum Planch. Indian Journal of Natural Sciences. 13 (75):1-12
  6. Kumar P*, Mahato DK, Gupta A, Pandhi S, Mishra S, Barua S, Tyagi V, Kumar A, Kumar M and Kamle M. (2022), Use of essential oils and phytochemicals against the mycotoxins producing fungi for shelf-life enhancement and food preservation. Int J Food Sci Technol. 57:2171–2184. https://doi.org/10.1111/ijfs.15563 (IF 3.612).
  7. Kamle M, Mahato DK, Gupta A, Pandhi S, Sharma B, Dhawan K, Vasundhara, Mishra S, Kumar M, Tripathi AD, Rasane P, Selvakumar R, Kumar A, Gamlath S, Kumar P*. Deoxynivalenol: An Overview on Occurrence, Chemistry, Biosynthesis, Health Effects and Its Detection, Management, and Control Strategies in Food and Feed. Microbiology Research. 2022; 13(2):292-314. (IF 1.5).
  8. Kamle M, Mahato DK, Gupta A, Pandhi S, Sharma N, Sharma B, Mishra S, Arora S, Selvakumar R, Saurabh V, Dhakane-Lad J, Kumar M, Barua S, Kumar A, Gamlath S, Kumar P*. (2022) Citrinin Mycotoxin Contamination in Food and Feed: Impact on Agriculture, Human Health, and Detection and Management Strategies. Toxins. 2022; 14(2):85. (IF 4.54).
  9. Mahato DK, Pandhi S, Kamle M, Gupta A, Sharma B, Panda BK, Srivastava S, Kumar M, Selvakumar R, Pandey AK, Suthar P, Arora S, Kumar A, Gamlath S, Bharti A, Kumar P.* (2022) Trichothecenes in food and feed: Occurrence, impact on human health and their detection and management strategies. Toxicon. 208:62-77. doi: 10.1016/j.toxicon.2022.01.011. (IF 3.035)
  10. Kumar S, Singh N, Devi LS, Kumar S, Kamle M, Kumar P*, Mukherjee A (2022) Neem oil and its nanoemulsion in sustainable food preservation and packaging: Current status and future prospects. Journal of Agriculture and Food Research 7: 100254. (IF 3.8).

Book Chapters

  1. Kamle M, Gogoi P, Mahato KD, Gupta A, Kumar P (2023) Food Preservation by Coating Technology: Nanotechnology Approach. In edited by Santosh Kumar, Avik Mukherjee, Atanu Mitra, Dipankar Halder (eds) Emerging Technologies in Food Preservation, 331-345.
  2. Sharma C, Kamle M, Kumar P (2023) Green Synthesis of Nanoparticles Using Various Plant Parts and Their Antifungal Activity. In Upadhyay SK, Singh SP (eds.) Plants as Bioreactors for Industrial Molecules. https://doi.org/10.1002/9781119875116.ch16
  3. Srivastava S, Yadav AK, Ghosh M, Mahato DK, Kamle M, Pandey P, Chakraborty S, Kumar P (2023) Fumonisins in Food and Feed: Their Detection and Management Strategies. In Kumar P, Kamle M, Mahato DK (eds). Mycotoxins in Food and Feed, CRC Press, USA. 29-49.
  4. Selvakumar R, Manjunathagowda DC, Pandey AK, Mahato DK, Gupta A, Pandhi S, Kargwal R, Kamle M, Kumar P (2023) Detection and Management Strategies for Deoxynivalenol in Food and Feed: An Overview (2023) In Kumar P, Kamle M, Mahato DK (eds). Mycotoxins in Food and Feed, CRC Press, USA. 119-155.
  5. Pandey AK, Vashishth R, Mahato DK, Kamle M, Selvakumar R, Mathur M, Kumar P (2023) Ochratoxins in Food and Feed: Detection and Management Strategies. In Kumar P, Kamle M, Mahato DK (eds). Mycotoxins in Food and Feed, CRC Press, USA. 51-85.
  6. Mathur M, Kargwal R, Selvakumar R, Mahato DK, Kamle M, Kumar P (2023) Climate Change’s Impact on Mycotoxin Production in Food and Feed. In Kumar P, Kamle M, Mahato DK (eds). Mycotoxins in Food and Feed, CRC Press, USA. 361-428.
  7. Ghosh M, Chakraborty S, Misra S, Srivastava S, Bondre S, Mahato DK, Kamle M, Kumar P (2023) PR Toxins: Concerns in Food and Feed with Their Detection and Management Strategies. In Kumar P, Kamle M, Mahato DK (eds). Mycotoxins in Food and Feed, CRC Press, USA. 239-264.
  8. Sharma C, Gogoi P, Kamle M, Kumar P* (2023) Nanocurcumin: Herbal trends to develop novel antifungal agents. In. Nanofungicides: Novel Applications in Plant Disease Control. By Kamel A Abd-Elsalam (ed.), ISBN: 9780323953054
  9. Sharma C, Gogoi P, Kamle M, Kumar P(2023) Applications of Nanoparticles and Nanomaterials in Bioremediation for Environmental Safety: An Overview. In Nanoparticles as Sustainable Environmental Remediation Agents. Konstantinos S & Stefanos M (Eds.). By Royal Society of Chemistry, USA, pp.1-28. 

Book

  1. Mycotoxins in Food and Feed: Detection and Management Strategies (2023)-Edited By Pradeep Kumar, Madhu Kamle, Dipendra Kumar Mahato. CRC Press (Taylor’s & Francis group) ISBN: 9781032113920